Studies on Independent Synthesis of Cytoplasmic Ribonucleic Acids in Acetabularia mediterranea
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منابع مشابه
Studies on Independent Synthesis of Cytoplasmic Ribonucleic Acids in Acetabularia mediterranea
1. The RNA content of anucleate and nucleate fragments of Acetabularia has been measured. It was found that there is a net synthesis of RNA in nucleate fragments. On the other hand, the RNA content of anucleate fragments did not change significantly after enucleation. 2. Anucleate fragments, however, can readily incorporate (14)C-labeled adenine, orotic acid, and carbon dioxide into their cytop...
متن کاملNucleic acid synthesis in the chloroplasts of Acetabularia mediterranea.
Radioautographic and radiochemical techniques were used to establish the presence of replicating DNA in the chloroplasts of Acetabularia mediterranea. These techniques also demonstrated that these chloroplasts synthesize RNA. It was found that label from thymine was also incorporated into DNA and RNA in these chloroplasts. With the establishment of protein and nucleic acid synthesis in Acetabul...
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The cytoplasm of an Acetabularia cell is normally at a potential of about -170 mv relative to the external solution; the vacuole is also at this potential. Although there is strict flux equilibrium for all ions, the potential is more negative than the Nernst potentials of any of the permeating ions. Darkness, CCCP, low temperature, and reducing [Cl(-)](o) by a factor of 25 all rapidly depolariz...
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The structural organization of transcriptionally active DNA that contains cistrons for precursor molecules of ribosomal RNA is described in positively stained spread preparations from nuclei and nucleoli isolated from the green alga, Acetabularia mediterranea Lmx. These nuclei contain large aggregates of nucleolar subunits in which fibril-covered regions, the putative active cistrons for precur...
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Rats infected with virulent Diplococcuspneumoniae developed a significant increase in the rate of incorporation of labelled orotate into hepatic RNA when compared with pair-fed controls inoculated with heat-killed organisms. The finding was readily detected in rats raised on either a low-protein diet (6% casein) or a diet containing adequate amounts of protein (18% casein). The increase in hepa...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1960
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.43.6.1083